Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H2O2

Cell Mol Biol Lett. 2019 Jun 17:24:42. doi: 10.1186/s11658-019-0169-6. eCollection 2019.

Abstract

Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H2O2. Apoptosis of HBE-Dp71AS cells induced by H2O2 was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinase B (p-AKT) were detected in the HBE-Dp71AS cells, which functioned together with RAD51 as the molecular explanations for the character alterations of HBE-Dp71AS cells.

Keywords: Apoptosis; DNA damage; Dp71; FAK; RAD51.

MeSH terms

  • Apoptosis*
  • Cell Line
  • DNA / drug effects
  • DNA / metabolism
  • DNA Damage*
  • DNA Repair
  • Dystrophin / metabolism*
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Focal Adhesion Kinase 1 / genetics
  • Focal Adhesion Kinase 1 / metabolism
  • Gene Expression Regulation
  • Humans
  • Hydrogen Peroxide / pharmacology
  • Hydrogen Peroxide / toxicity*
  • Lamin Type B / genetics
  • Oxidative Stress*
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Rad51 Recombinase / genetics*
  • Rad51 Recombinase / metabolism

Substances

  • Dystrophin
  • Lamin Type B
  • apo-dystrophin 1
  • DNA
  • Hydrogen Peroxide
  • Focal Adhesion Kinase 1
  • PTK2 protein, human
  • RAD51 protein, human
  • Rad51 Recombinase