Carbogenic Nanozyme with Ultrahigh Reactive Nitrogen Species Selectivity for Traumatic Brain Injury

Nano Lett. 2019 Jul 10;19(7):4527-4534. doi: 10.1021/acs.nanolett.9b01333. Epub 2019 Jun 20.

Abstract

Reactive oxygen and nitrogen species (RONS), especially reactive nitrogen species (RNS) are intermediate products during incidence of nervous system diseases, showing continuous damage for traumatic brain injury (TBI). Here, we developed a carbogenic nanozyme, which shows an antioxidant activity 12 times higher than ascorbic acid (AA) and behaves as multienzyme mimetics. Importantly, the nanozyme exhibits an ultrahigh scavenging efficiency (∼16 times higher than AA) toward highly active RNS, such as •NO and ONOO- as well as traditional reactive oxygen species (ROS) including O2•-, H2O2, and •OH. In vitro experiments show that neuron cells injured by H2O2 or lipopolysaccharide can be significantly recovered after carbogenic nanozyme treatment via scavenging all kinds of RONS. Moreover, the carbogenic nanozyme can serve as various enzyme mimetics and eliminate the harmful peroxide and glutathione disulfide from injured mice, demonstrating its potential as a therapeutic for acute TBI.

Keywords: Traumatic brain injury; catalytic selectivity; nanozyme; reactive oxygen and nitrogen species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Biomimetic Materials* / chemistry
  • Biomimetic Materials* / pharmacology
  • Brain Injuries, Traumatic* / drug therapy
  • Brain Injuries, Traumatic* / metabolism
  • Brain Injuries, Traumatic* / pathology
  • Disease Models, Animal
  • Glutathione Disulfide / metabolism*
  • Hydrogen Peroxide / metabolism*
  • Mice
  • Nanostructures* / chemistry
  • Nanostructures* / therapeutic use
  • Reactive Nitrogen Species / metabolism*

Substances

  • Reactive Nitrogen Species
  • Hydrogen Peroxide
  • Glutathione Disulfide