Apigenin Exerts Anti-inflammatory Effects in an Experimental Model of Acute Pancreatitis by Down-regulating TNF-α

In Vivo. 2019 Jul-Aug;33(4):1133-1141. doi: 10.21873/invivo.11583.

Abstract

Background/aim: This study investigated the anti-inflammatory effect of apigenin in an experimental model of acute pancreatitis. Inflammatory response was reflected by tissue expression of the cytokine TNF-α coupled with histological examination.

Materials and methods: Wistar rats were divided into three groups: Sham-group animals underwent laparotomy only, without any other interventions. Control-group animals underwent laparotomy and bilio-pancreatic duct ligation to induce pancreatitis without apigenin administration. Apigenin group animals were further treated with apigenin. Euthanasia was performed at 6, 12, 24, 48 and 72 h post-operatively.

Results: Over-expression of TNF-α in relation to postoperative time was observed in the control group (p<0.001). In the apigenin group, under-expression of TNF-α in relation to postoperative time was observed (p<0.013). At 72 h, apigenin reduced pancreatic TNF-α expression and prevented pancreatic necrosis.

Conclusion: Apigenin slows progression and reduces severity of acute pancreatitis. Apigenin may serve as an adjunct to a more successful therapeutic strategy in acute pancreatitis.

Keywords: Apigenin; TNF-α; acute pancreatitis; biliopancreatic duct ligation.

MeSH terms

  • Acute Disease
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Apigenin / pharmacology*
  • Biomarkers
  • Immunohistochemistry
  • Male
  • Pancreatitis / drug therapy
  • Pancreatitis / etiology
  • Pancreatitis / metabolism*
  • Pancreatitis / pathology
  • Rats
  • Time Factors
  • Tumor Necrosis Factor-alpha / metabolism*

Substances

  • Anti-Inflammatory Agents
  • Biomarkers
  • Tumor Necrosis Factor-alpha
  • Apigenin