Cell-wall associated polysaccharide from the psychrotolerant bacterium Psychrobacter arcticus 273-4: isolation, purification and structural elucidation

Extremophiles. 2020 Jan;24(1):63-70. doi: 10.1007/s00792-019-01113-8. Epub 2019 Jul 15.

Abstract

In this paper, the structure of the capsular polysaccharide isolated from the psychrotolerant bacterium Psychrobacter arcticus 273-4 is reported. The polymer was purified by gel filtration chromatography and the structure was elucidated by means of one- and two-dimensional NMR spectroscopy, in combination with chemical analyses. The polysaccharide consists of a trisaccharidic repeating unit containing two residues of glucose and a residue of a N,N-diacetyl-pseudaminic acid.

Keywords: Capsular polysaccharide; Extracellular polysaccharide (EPS); Pseudaminic acid; Psychrotolerant.

MeSH terms

  • Cell Wall
  • Magnetic Resonance Spectroscopy
  • Polysaccharides
  • Psychrobacter*

Substances

  • Polysaccharides

Supplementary concepts

  • Psychrobacter arcticus