Multiscale Genome Engineering: Genome-Wide Screens and Targeted Approaches

Neville E. Sanjana

doi:10.1007/978-3-319-60192-2_8

Multiscale Genome Engineering: Genome-Wide Screens and Targeted Approaches

Review

In: Genome Editing in Neurosciences [Internet]. Cham (CH): Springer; 2017.

2017 Sep 15.

Author

Neville E. Sanjana^{1

2}

Book Editors

Rudolf Jaenisch¹, Feng Zhang², Fred Gage³

Affiliations

¹ New York Genome Center, New York, NY, USA
² Department of Biology, New York University, New York, NY, USA

Book Affiliations

¹ Whitehead Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
² Department of Brain and Cognitive Science, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
³ Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, California, USA

Excerpt

New advances in genome engineering technologies, such as efficient programmable CRISPR nucleases, have enabled new advances in forward and reverse genetic studies. Here, I discuss recent work from our group combining top-down approaches like genome-wide loss-of-function screens and bottom-up approaches like disease variant modeling in human stem cells and stem cell-derived cortical neurons.

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Review