Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity

Mirian Michelle Machado De-Paula; Samson Afewerki; Bartolomeu Cruz Viana; Thomas Jay Webster; Anderson Oliveira Lobo; Fernanda Roberta Marciano

doi:10.1016/j.msec.2019.109778

Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity

Mater Sci Eng C Mater Biol Appl. 2019 Oct:103:109778. doi: 10.1016/j.msec.2019.109778. Epub 2019 May 21.

Authors

Mirian Michelle Machado De-Paula¹, Samson Afewerki², Bartolomeu Cruz Viana³, Thomas Jay Webster⁴, Anderson Oliveira Lobo⁵, Fernanda Roberta Marciano⁶

Affiliations

¹ University of Vale do Paraiba, 12244-000 Sao Jose dos Campos, SP, Brazil.
² Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
³ Department of Physics and Materials Science and Engineering graduate program, Federal University of Piaui, 64049-550 Teresina, PI, Brazil.
⁴ Nanomedicine Laboratory, Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA. Electronic address: th.webster@neu.edu.
⁵ Interdisciplinary Laboratory for Advanced Materials, Department of Materials Engineering, Federal University of Piaui, 64049-550 Teresina, PI, Brazil. Electronic address: lobo@ufpi.edu.br.
⁶ Department of Physics and Materials Science and Engineering graduate program, Federal University of Piaui, 64049-550 Teresina, PI, Brazil. Electronic address: femarciano@gmail.com.

PMID: 31349506
DOI: 10.1016/j.msec.2019.109778

Abstract

Herein, we electrospun ultrathin core-shell fibers based on polycaprolactone (PCL), polyethylene glycol (PEG), gelatin and osteogenic growth peptide (OGP), and evaluated their potential to upregulate human osteoblast cells (hFOB) and to reduce Gram-positive and Gram-negative bacteria. We also evaluated the fiber morphology, chemical structure and peptide delivery efficacy. The employment of core-shell fibers compared to fibers without a core-shell showed improved mechanical strength, comparable to the strength of pure PCL, as well as improved hydrophilicity and wettability. The careful selection of polymer combination and core-shell strategy promoted a controlled and sustained release of OGP. Moreover, increased calcium deposition (CD) (1.3-fold) and alkaline phosphate (ALP) activity was observed when hFOBs were cultivated onto core-shell fibers loaded with OGP after 21 days of culture. Our developed scaffolds were also able to reduce the amount of Pseudomonas aeruginosa (ATCC 25668) bacteria by a factor of two compared to raw PCL without the use of any antibiotics. All of these results demonstrate a promising potential of the developed core-shell electrospun scaffolds based on PCL:PEG:Gelatin:OGP for numerous bone tissue applications.

Keywords: Bactericidal effect; Bone regeneration; Core-shell; Electrospinning; Osteogenic growth peptide; PCL.

MeSH terms

Cell Differentiation / drug effects*
Cell Line
Histones* / chemistry
Histones* / pharmacology
Humans
Intercellular Signaling Peptides and Proteins* / chemistry
Intercellular Signaling Peptides and Proteins* / pharmacology
Methicillin-Resistant Staphylococcus aureus / growth & development*
Osteoblasts / metabolism*
Pseudomonas aeruginosa / growth & development*
Tissue Engineering*
Tissue Scaffolds / chemistry*

Substances

Histones
Intercellular Signaling Peptides and Proteins
osteogenic growth peptide