Extracellular Vesicles with Exosome-like Features Transfer TLRs between Dendritic Cells

Immunohorizons. 2019 Jun 4;3(6):186-193. doi: 10.4049/immunohorizons.1900016.

Abstract

Accumulating evidence shows that extracellular vesicles (EVs) secreted by immune cells play an important role in intercellular communication. In the current report, we show that EVs released from wild-type bone marrow-derived dendritic cells (BMDCs) transfer TLRs to TLR4-knockout (TLR4KO) BMDCs and increase cellular responsiveness to LPS in recipient cells. The transferred EVs have exosomal characteristics and induce the activation of NF-κB signaling pathways in recipient cells. We further show that BMDC-derived EVs can promote LPS-induced inflammation in TLR4KO mice in vivo. These results indicate that functional TLR4 can be transferred from wild-type to TLR4KO BMDCs through exosome-like EVs.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Dendritic Cells / immunology*
  • Exosomes / metabolism*
  • Exosomes / pathology
  • Extracellular Vesicles / metabolism*
  • Extracellular Vesicles / pathology
  • Inflammation / immunology*
  • Lipopolysaccharides / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NF-kappa B / metabolism
  • Signal Transduction
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / metabolism*

Substances

  • Lipopolysaccharides
  • NF-kappa B
  • Toll-Like Receptor 4