Exogenous polyamines enhance resistance to Alternaria alternata by modulating redox homeostasis in apricot fruit

Yongcai Li; Yueyue Ma; Tingting Zhang; Yang Bi; Yi Wang; Dov Prusky

doi:10.1016/j.foodchem.2019.125303

Exogenous polyamines enhance resistance to Alternaria alternata by modulating redox homeostasis in apricot fruit

Food Chem. 2019 Dec 15:301:125303. doi: 10.1016/j.foodchem.2019.125303. Epub 2019 Jul 31.

Authors

Yongcai Li¹, Yueyue Ma², Tingting Zhang², Yang Bi³, Yi Wang⁴, Dov Prusky⁵

Affiliations

¹ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: lyc@gsau.edu.cn.
² College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
³ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: biyang@gsau.edu.cn.
⁴ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: wangyi@gsau.edu.cn.
⁵ College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China; Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Beit Dagan, Israel. Electronic address: dovprusk@agri.gov.il.

PMID: 31387045
DOI: 10.1016/j.foodchem.2019.125303

Abstract

The effects of exogenous polyamines treatment on reactive oxygen species (ROS) metabolism in apricot fruits were systematically analyzed through the investigation of their curative and preventive effects on black spot disease. Results showed that 1.5 mM spermine (Spm), 1.5 mM spermidine (Spd) and 10 mM putrescine (Put) treatment significantly inhibited black spot development, additionally, the efficacy of this control was dependent upon the type of polyamines used and concentration level applied. Further studies have shown that exogenous polyamines treatments significantly improved production of O₂^- and H₂O₂, and increased the activities and gene expression levels of NADPH oxidase (NOX), super oxide dismutase (SOD), catalase (CAT) ascorbate peroxidase (AXP) and glutathione reductase (GR) in apricot fruit. Increased ascorbic acid (AsA) and reduced glutathione (GSH) content were also observed after exogenous polyamines treatment. These results have revealed that postharvest polyamines treatment effectively enhanced disease resistance through the maintenance of homeostasis in apricot fruits.

Keywords: Apricot fruit; Black spot; Exogenous polyamines; Induced resistance; ROS metabolism.

MeSH terms

Alternaria / pathogenicity*
Ascorbate Peroxidases / metabolism
Catalase / metabolism
Disease Resistance / drug effects
Fruit / drug effects*
Fruit / metabolism
Fruit / microbiology
Glutathione / metabolism
Glutathione Reductase / metabolism
Homeostasis / drug effects
Hydrogen Peroxide / metabolism
Oxidation-Reduction
Plant Diseases / microbiology*
Polyamines / pharmacology*
Prunus armeniaca / drug effects*
Prunus armeniaca / metabolism
Prunus armeniaca / microbiology
Reactive Oxygen Species / metabolism
Superoxide Dismutase / metabolism

Substances

Polyamines
Reactive Oxygen Species
Hydrogen Peroxide
Ascorbate Peroxidases
Catalase
Superoxide Dismutase
Glutathione Reductase
Glutathione