PAK4 suppresses RELB to prevent senescence-like growth arrest in breast cancer

Nat Commun. 2019 Aug 9;10(1):3589. doi: 10.1038/s41467-019-11510-4.

Abstract

Overcoming cellular growth restriction, including the evasion of cellular senescence, is a hallmark of cancer. We report that PAK4 is overexpressed in all human breast cancer subtypes and associated with poor patient outcome. In mice, MMTV-PAK4 overexpression promotes spontaneous mammary cancer, while PAK4 gene depletion delays MMTV-PyMT driven tumors. Importantly, PAK4 prevents senescence-like growth arrest in breast cancer cells in vitro, in vivo and ex vivo, but is not needed in non-immortalized cells, while PAK4 overexpression in untransformed human mammary epithelial cells abrogates H-RAS-V12-induced senescence. Mechanistically, a PAK4 - RELB - C/EBPβ axis controls the senescence-like growth arrest and a PAK4 phosphorylation residue (RELB-Ser151) is critical for RELB-DNA interaction, transcriptional activity and expression of the senescence regulator C/EBPβ. These findings establish PAK4 as a promoter of breast cancer that can overcome oncogene-induced senescence and reveal a selective vulnerability of cancer to PAK4 inhibition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Breast / cytology
  • Breast / pathology
  • Breast Neoplasms / mortality
  • Breast Neoplasms / pathology*
  • Cell Line, Tumor
  • Cellular Senescence / genetics
  • Epithelial Cells
  • Female
  • Gene Knockdown Techniques
  • Humans
  • Mammary Glands, Animal / pathology
  • Mammary Neoplasms, Experimental / genetics
  • Mammary Neoplasms, Experimental / pathology
  • Mice
  • Mice, Transgenic
  • Middle Aged
  • Primary Cell Culture
  • Prognosis
  • Transcription Factor RelB / metabolism*
  • Xenograft Model Antitumor Assays
  • p21-Activated Kinases / genetics
  • p21-Activated Kinases / metabolism*

Substances

  • RELB protein, human
  • Transcription Factor RelB
  • PAK4 protein, human
  • Pak4 protein, mouse
  • p21-Activated Kinases