Phenomenon of atypical vascular effects of epinephrine and an increase of photodynamic response by nitroglycerin in rats with colon adenocarcinoma: adrenergic and nitrergic mechanisms and novel applied aspects

Ilana Agranovich; Ekaterina Borisova; Nikita Navolokin; Alla Bucharskaya; Galina Maslyakova; Alexander Shirokov; Arkady Abdurashitov; Ivan Angelov; Alexander Khorovodov; Andrey Terskov; Aysel Mamedova; Maria Klimova; Oxana Semyachkina-Glushkovskaya

doi:10.1364/BOE.10.004115

Phenomenon of atypical vascular effects of epinephrine and an increase of photodynamic response by nitroglycerin in rats with colon adenocarcinoma: adrenergic and nitrergic mechanisms and novel applied aspects

Biomed Opt Express. 2019 Jul 18;10(8):4115-4125. doi: 10.1364/BOE.10.004115. eCollection 2019 Aug 1.

Authors

Affiliations

¹ Saratov State University, Astrakhanskaya Str. 83, Saratov 410012, Russia.
² Institute of Electronics, Bulgarian Academy of Sciences, Tsarigradsko Chaussee Blvd. 72, Sofia 1784, Bulgaria.
³ Saratov State Medical University, Bolshaya Kazachia str. 112, Saratov 410012, Russia.
⁴ Institute of Bioorganic Chemistry, Russian Academy of Sciences, Entusiastov Str. 13, Saratov 410049, Russia.
⁵ Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bontchev str. Bl. 9,1113 Sofia, Bulgaria.

Abstract

Using an original model of stress-induced colon adenocarcinoma, we uncover atypical vasorelaxation effects of a mucosa injection of epinephrine assessed by laser speckle contrast imaging and a significant increase of fluorescent intensity of 5-ALA/PpIX from malignant colon tissues by a mucosa injection of nitroglycerine. We clearly demonstrate a high activity of adrenergic and nitrergic mechanisms underlying this phenomenon and discuss their application in improving of optical approaches for effective diagnosis of gastrointestinal cancer.