Effect of RNA Interference on the Hepatitis C Virus Core Expression in HepG2/C3A Cells Using Genotype 4 Isolates from Saudi Patients

J Coll Physicians Surg Pak. 2019 Sep;29(9):852-859. doi: 10.29271/jcpsp.2019.09.852.

Abstract

Objective: To explore the effects of two different methodologies of RNA interference, namely small interfering RNA, and vector-based short hairpin RNA, on the expression levels of hepatitis C virus core RNA and protein of Saudi genotype 4 isolates.

Study design: An experimental study.

Place and duration of study: Laboratories of the College of Medicine Research Center, King Saud University, Saudi Arabia, from January to December 2018.

Methodology: Hepatitis C virus core small interfering RNA molecule and short hairpin RNA vector were designed against core region. Viral RNA expression was tested by RT-PCR; whereas, core protein was tested by flow cytometry and immunofluorescence. Results were statistically analysed by Chi-square analysis to calculate the p-value.

Results: Both molecules caused a reduction in core RNA and protein expression in infected cells. The effect of 100-pmol of small interfering RNA was more evident. For the vector-based short hairpin RNA, inhibition of core RNA expression was quite evident after 96 hours (p = 0.007). The results of flow cytometry and immunofluorescence showed a decline in core protein expression. The most dramatic effect was observed with 100-pmol small interfering RNA treatment of cells for 24 and 48 hours, which resulted in 63.5% and 91.1% core RNA expression reduction, respectively.

Conclusion: RNA interference of hepatitis C virus core gene efficiently stopped viral replication and offer a promising therapeutic alternative against virus infection.

MeSH terms

  • Cell Culture Techniques
  • Hep G2 Cells
  • Hepacivirus / growth & development*
  • Humans
  • RNA Interference*
  • RNA, Small Interfering*
  • RNA, Viral*
  • Saudi Arabia
  • Viral Core Proteins / metabolism*
  • Virus Replication*

Substances

  • RNA, Small Interfering
  • RNA, Viral
  • Viral Core Proteins