Hyb-Seq for Flowering Plant Systematics

Steven Dodsworth; Lisa Pokorny; Matthew G Johnson; Jan T Kim; Olivier Maurin; Norman J Wickett; Felix Forest; William J Baker

doi:10.1016/j.tplants.2019.07.011

Hyb-Seq for Flowering Plant Systematics

Trends Plant Sci. 2019 Oct;24(10):887-891. doi: 10.1016/j.tplants.2019.07.011. Epub 2019 Aug 30.

Authors

Steven Dodsworth¹, Lisa Pokorny², Matthew G Johnson³, Jan T Kim⁴, Olivier Maurin⁴, Norman J Wickett⁵, Felix Forest⁴, William J Baker⁴

Affiliations

¹ Royal Botanic Gardens, Kew, Richmond TW9 3AE, Surrey, UK; School of Life Sciences, University of Bedfordshire, University Square, Luton LU1 3JU, UK. Electronic address: steven.dodsworth@beds.ac.uk.
² Royal Botanic Gardens, Kew, Richmond TW9 3AE, Surrey, UK; Centre for Plant Biotechnology and Genomics (CBGP, UPM-INIA), 28223 Pozuelo de Alarcón, Madrid, Spain.
³ Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA; Chicago Botanic Garden, Glencoe, IL 60022, USA.
⁴ Royal Botanic Gardens, Kew, Richmond TW9 3AE, Surrey, UK.
⁵ Chicago Botanic Garden, Glencoe, IL 60022, USA; Program in Plant Biology and Conservation, Northwestern University, Evanston, IL 60208, USA.

PMID: 31477409
DOI: 10.1016/j.tplants.2019.07.011

Abstract

High-throughput DNA sequencing (HTS) presents great opportunities for plant systematics, yet genomic complexity needs to be reduced for HTS to be effectively applied. We highlight Hyb-Seq as a promising approach, especially in light of the recent development of probes enriching 353 low-copy nuclear genes from any flowering plant taxon.

Keywords: Hyb-Seq; angiosperms; genomics; high-throughput sequencing; molecular systematics; phylogenetics; sequence capture; tree of life.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Nucleus
Genomics
High-Throughput Nucleotide Sequencing
Magnoliopsida*
Phylogeny
Sequence Analysis, DNA