Comparative Antibiofilm Efficacy of Meropenem Alone and in Combination with Colistin in an In Vitro Pharmacodynamic Model by Extended-Spectrum-β-Lactamase-Producing Klebsiella pneumoniae

Antimicrob Agents Chemother. 2019 Oct 22;63(11):e01230-19. doi: 10.1128/AAC.01230-19. Print 2019 Nov.

Abstract

We compared the efficacies of meropenem alone and in combination with colistin against two strains of extended-spectrum-β-lactamase-producing Klebsiella pneumoniae, using an in vitro pharmacodynamic model that mimicked two different biofilm conditions. Meropenem monotherapy achieved remarkable efficacy (even a bactericidal effect) under all conditions, whereas colistin was almost inactive and resistance emerged. The addition of colistin to meropenem produced no relevant benefits, in contrast to experiences with other microorganisms.

Keywords: Klebsiella pneumoniae; biofilms; colistin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / pharmacology*
  • Biofilms / drug effects*
  • Colistin / administration & dosage
  • Colistin / pharmacology*
  • Drug Resistance, Multiple, Bacterial
  • Drug Therapy, Combination
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / ultrastructure
  • Meropenem / administration & dosage
  • Meropenem / pharmacology*
  • Microscopy, Electron, Scanning
  • beta-Lactam Resistance
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • beta-Lactamases
  • Meropenem
  • Colistin