Place navigation in the Morris water task results in greater nuclear Arc mRNA expression in dorsal compared to ventral CA1

Hippocampus. 2019 Nov;29(11):1133-1138. doi: 10.1002/hipo.23157. Epub 2019 Sep 11.

Abstract

Previous work has shown that the dorsal hippocampus has greater activity than ventral regions during place navigation. Exposure to a novel context has also been found to increase hippocampal activation, possibly due to increased spatial demands. However, activation patterns in dorsal and ventral regions have not been investigated in the Morris water task (MWT), which remains the most popular assay of place memory in rodents. We measured activity in a large population of neurons across the CA1 dorsal-ventral axis by estimating nuclear Arc mRNA with stereologic systematic-random sampling procedures following changes to goal location or spatial context in the MWT in rats. Following changes to goal location or spatial context in the MWT, we did not find an effect on Arc mRNA expression in CA1. However, Arc expression was greater in the dorsal compared to the ventral aspect of CA1 during task performance. Several views might account for these observed differences in dorsal-ventral Arc mRNA expression, including task parameters or the granularity of representation that differs along the dorsal-ventral hippocampal axis. Future work should determine the effects of task differences and required memory precision in relation to dorsal-ventral hippocampal neuronal activity.

Keywords: Arc; Morris water task; dorsal; hippocampus; ventral.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CA1 Region, Hippocampal / metabolism*
  • Cell Nucleus / metabolism*
  • Cytoskeletal Proteins / biosynthesis*
  • Cytoskeletal Proteins / genetics
  • Maze Learning / physiology*
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • Rats

Substances

  • Cytoskeletal Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • activity regulated cytoskeletal-associated protein