Modulation of the fungal mycobiome is regulated by the chitin-binding receptor FIBCD1

J Exp Med. 2019 Dec 2;216(12):2689-2700. doi: 10.1084/jem.20182244. Epub 2019 Oct 10.

Abstract

Host-microbiota interactions are critical in regulating mammalian health and disease. In addition to bacteria, parasites, and viruses, beneficial communities of fungi (the mycobiome) are important modulators of immune- and tissue-homeostasis. Chitin is a major component of the fungal cell wall, and fibrinogen C containing domain 1 (FIBCD1) is a chitin-binding protein; however, the role of this molecule in influencing host-mycobiome interactions in vivo has never been examined. Here, we identify direct binding of FIBCD1 to intestinal-derived fungi and demonstrate that epithelial-specific expression of FIBCD1 results in significantly reduced fungal colonization and amelioration of fungal-driven intestinal inflammation. Collectively, these results identify FIBCD1 as a previously unrecognized microbial pattern recognition receptor through which intestinal epithelial cells can recognize and control fungal colonization, limit fungal dysbiosis, and dampen intestinal inflammation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chitin / metabolism
  • DNA, Ribosomal Spacer
  • Disease Models, Animal
  • Enteritis / etiology
  • Enteritis / metabolism
  • Enteritis / pathology
  • Fungi / physiology*
  • Gastrointestinal Microbiome
  • Gene Expression
  • Humans
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / microbiology
  • Intestinal Mucosa / pathology
  • Metagenomics
  • Mice
  • Mice, Transgenic
  • Microbial Interactions*
  • Mycobiome*
  • Protein Binding
  • RNA, Ribosomal, 16S
  • Receptors, Cell Surface / metabolism*

Substances

  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 16S
  • Receptors, Cell Surface
  • Chitin