Fabrication of Chitosan/PVA/GO/CuO patch for potential wound healing application

Int J Biol Macromol. 2020 Jan 15:143:744-762. doi: 10.1016/j.ijbiomac.2019.10.029. Epub 2019 Oct 14.

Abstract

Wound healing is a common issue in our day to day life. Our immune system repairs the damaged tissue by itself and its a time-consuming process. The GO/CuO nanocomposite (NC) was synthesized through the sol-gel method. XRD, FT-IR, Raman, and TEM analysis were used to analysis the physico-chemical properties of the sample. The GO/CuO patches were prepared using chitosan (Cs)/poly vinyl alcohol (PVA) due to its biocompatibility and biodegradable nature. The obtained patches showed better antimicrobial and wound healing property than recently reported materials. The GO/CuO NC plays a major part in angiogenesis process and in the synthesis, stabilization of extracellular matrix skin proteins. Thus, GO/CuO NC enhance the wound healing mechanism by increasing cell proliferation, antimicrobial property and rapid initiation of inflammatory. Moreover, the antimicrobial activity of CuO, GO, GO/CuO and GO/CuO patch were tested against bacteria causing wound infections. Cs/PVA patch and Cs/PVA/GO/CuO patch were analyzed for swelling, evaporation and degradation behavior. Increase in cell viability and migration of NIH3t3 cells by NC patch shows a potential way for wound healing applications.

Keywords: Antimicrobial activity; Chitosan; Wound healing.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Bacteria / drug effects
  • Bacteria / ultrastructure
  • Cell Movement / drug effects
  • Cell Survival / drug effects
  • Chitosan / pharmacology*
  • Copper / pharmacology*
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Graphite / pharmacology*
  • Humans
  • Inhibitory Concentration 50
  • Mice
  • Microbial Sensitivity Tests
  • NIH 3T3 Cells
  • Polyvinyl Alcohol / chemistry
  • Polyvinyl Alcohol / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Wound Healing / drug effects*
  • X-Ray Diffraction

Substances

  • Anti-Bacterial Agents
  • Reactive Oxygen Species
  • graphene oxide
  • Graphite
  • Copper
  • Polyvinyl Alcohol
  • Chitosan
  • cupric oxide