Exercise does not ameliorate cardiac dysfunction in obese mice exposed to fine particulate matter

Abstract

Background: Studies have demonstrated that exposure to fine particulate matter (PM_2.5) is linked to cardiovascular disease (CVD), which is exacerbated in patients with pre-existing conditions such as obesity. In the present study, we examined cardiac function of obese mice exposed to PM_2.5 and determined if mild exercise affected cardiac function.

Methods: Obese mice (ob/ob) (leptin deficient, C57BL/6J background) were exposed to either filtered air (FA) or PM_2.5 at an average concentration of 32 μg/m³ for 6 h/day, 5 days/week for 9 months. Following exposure, mice were divided into four groups: (1) FA sedentary, (2) FA treadmill exercise, (3) PM_2.5 sedentary, and (4) PM_2.5 treadmill exercise and all mice were analyzed after 8 weeks of exercise training.

Results: Echocardiography showed increased left ventricular end systolic (LVESd) and diastolic (LVEDd) diameters in PM_2.5 sedentary mice compared to FA sedentary mice. There was increased expression of ICAM1, VCAM and CRP markers in sedentary PM_2.5 mice compared to FA mice. Both FA and PM_2.5 exercised mice showed decreased posterior wall thickness in systole compared to FA sedentary mice, coupled with altered expression of inflammatory markers following exercise.

Conclusion: Obese mice exposed to PM_2.5 for 9 months showed cardiac dysfunction, which was not improved following mild exercise training.

Keywords: Cardiovascular function; Exercise; Obesity; Particulate matter; Treadmill.