Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples

Tomohiro Haruta; Keiko Hasumi; Yuta Ikeda; Yuji Konyuba; Tomohisa Fukuda; Hideo Nishioka

doi:10.1093/jmicro/dfz031

Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples

Microscopy (Oxf). 2019 Dec 3;68(6):467-470. doi: 10.1093/jmicro/dfz031.

Authors

Tomohiro Haruta¹, Keiko Hasumi¹, Yuta Ikeda¹, Yuji Konyuba¹, Tomohisa Fukuda¹, Hideo Nishioka¹

Affiliation

¹ Application Management Department, JEOL Ltd, 3-1-2 Musashino, Akishima, Tokyo, Japan.

PMID: 31687748
DOI: 10.1093/jmicro/dfz031

Abstract

In this study, we investigated use of local surface plasmon resonance (LSPR) of metal nanoparticles (NPs) as a correlative light and electron microscopy (CLEM) tag for biological samples. Gold NPs in ultra-thin sections for TEM revealed that LSPR could be observed by optical microscopy at sizes of 20 nm or larger. Gold NPs at sizes less than 20 nm could be observed using the gold enhancement method. Therefore, this CLEM tag could be applied to immunoelectron microscopy using this gold enhancement method.

Keywords: Au NPs; LSPR; SiN film; correlative microscopy; imaging tag.

MeSH terms

Gold / chemistry*
Metal Nanoparticles / chemistry*
Microscopy, Electron, Transmission
Paramecium caudatum / ultrastructure
Particle Size
Surface Plasmon Resonance*

Substances

Gold