Human equilibrative nucleoside transporter-1 expression is a predictor in patients with resected pancreatic cancer treated with adjuvant S-1 chemotherapy

Cancer Sci. 2020 Feb;111(2):548-560. doi: 10.1111/cas.14258. Epub 2019 Dec 19.

Abstract

The high expression of human equilibrative nucleoside transporter-1 (hENT1) and the low expression of dihydropyrimidine dehydrogenase (DPD) are reported to predict a favorable prognosis in patients treated with gemcitabine (GEM) and 5-fluorouracil (5FU) as the adjuvant setting, respectively. The expression of hENT1 and DPD were analyzed in patients registered in the JASPAC 01 trial, which showed a better survival of S-1 over GEM as adjuvant chemotherapy after resection for pancreatic cancer, and their possible roles for predicting treatment outcomes and selecting a chemotherapeutic agent were investigated. Intensity of hENT1 and DPD expression was categorized into no, weak, moderate or strong by immunohistochemistry staining, and the patients were classified into high (strong/moderate) and low (no/weak) groups. Specimens were available for 326 of 377 (86.5%) patients. High expression of hENT1 and DPD was detected in 100 (30.7%) and 63 (19.3%) of 326 patients, respectively. In the S-1 arm, the median overall survival (OS) with low hENT1, 58.0 months, was significantly better than that with high hENT1, 30.9 months (hazard ratio 1.75, P = 0.007). In contrast, there were no significant differences in OS between DPD low and high groups in the S-1 arm and neither the expression levels of hENT1 nor DPD revealed a relationship with treatment outcomes in the GEM arm. The present study did not show that the DPD and hENT1 are useful biomarkers for choosing S-1 or GEM as adjuvant chemotherapy. However, hENT1 expression is a significant prognostic factor for survival in the S-1 arm.

Keywords: JASPAC 01; biomarker; dihydropyrimidine dehydrogenase; human equilibrative nucleoside transporter-1; pancreatic cancer.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antimetabolites, Antineoplastic / administration & dosage*
  • Antimetabolites, Antineoplastic / pharmacology
  • Biomarkers, Tumor / metabolism
  • Chemotherapy, Adjuvant
  • Clinical Trials, Phase I as Topic
  • Dihydrouracil Dehydrogenase (NADP) / metabolism
  • Drug Combinations
  • Equilibrative Nucleoside Transporter 1 / metabolism*
  • Female
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Male
  • Middle Aged
  • Oxonic Acid / administration & dosage*
  • Oxonic Acid / pharmacology
  • Pancreatectomy / methods*
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / therapy*
  • Prognosis
  • Randomized Controlled Trials as Topic
  • Retrospective Studies
  • Survival Analysis
  • Tegafur / administration & dosage*
  • Tegafur / pharmacology
  • Treatment Outcome

Substances

  • Antimetabolites, Antineoplastic
  • Biomarkers, Tumor
  • Drug Combinations
  • Equilibrative Nucleoside Transporter 1
  • SLC29A1 protein, human
  • S 1 (combination)
  • Tegafur
  • Oxonic Acid
  • Dihydrouracil Dehydrogenase (NADP)