Improved Physical Stability of an Antibody-Drug Conjugate Using Host-Guest Chemistry

Bioconjug Chem. 2020 Jan 15;31(1):123-129. doi: 10.1021/acs.bioconjchem.9b00809. Epub 2019 Dec 16.

Abstract

Antibody-drug conjugates (ADCs) are an emerging class of biopharmaceutical products for oncology, with the cytotoxic pyrrolobenzodiazepine (PBD) family of "warheads" well-established in the clinic. While PBDs offer high potency, they are also characterized by their hydrophobicity, which can make formulation of the ADC challenging. Several approaches have been investigated to improve the physicochemical properties of PBD-containing ADCs, and herein a supramolecular approach was explored using cucurbit[8]uril (CB[8]). The ability of CB[8] to simultaneously encapsulate two guests was exploited to incorporate a 12-mer polyethylene glycol harboring a methyl viologen moiety at one terminus (MV-PEG12), together with a PBD harboring an indole moiety at the C2' position (SG3811). This formulation approach successfully introduced a hydrophilic PEG to mask the hydrophobicity of SG3811, improving the physical stability of the ADC while avoiding any loss of potency related to chemical modification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzodiazepines / chemistry*
  • Bridged-Ring Compounds / chemistry*
  • Drug Stability
  • Hydrophobic and Hydrophilic Interactions
  • Imidazoles / chemistry*
  • Immunoconjugates / chemistry*
  • Polyethylene Glycols / chemistry
  • Pyrroles / chemistry*

Substances

  • Bridged-Ring Compounds
  • Imidazoles
  • Immunoconjugates
  • Pyrroles
  • cucurbit(8)uril
  • pyrrolo(2,1-c)(1,4)benzodiazepine
  • Benzodiazepines
  • Polyethylene Glycols