Evaluation of dengue serological tests available in Puerto Rico for identification of prior dengue infection for prevaccination screening

Diagn Microbiol Infect Dis. 2020 Mar;96(3):114918. doi: 10.1016/j.diagmicrobio.2019.114918. Epub 2019 Oct 24.

Abstract

We assessed a dengue IgG rapid diagnostic test (RDT) and IgG enzyme-linked immunosorbent assay (ELISA) to determine their suitability for dengue prevaccination screening. Each exhibited ≥99% specificity. The RDT demonstrated no Zika cross-reactivity, while the ELISA displayed greater sensitivity. Both could safely guide vaccination in Puerto Rico pending availability of improved serotests.

Keywords: CYD-TDV; Cross-reactivity; Dengue serostatus; Dengvaxia; Flaviviruses; Prevaccination screening; Prior dengue infection; Sensitivity; Specificity.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Viral / blood*
  • Clinical Trials as Topic
  • Cross Reactions / immunology
  • Dengue / blood
  • Dengue / diagnosis*
  • Dengue / immunology
  • Dengue Vaccines / administration & dosage
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Puerto Rico
  • Sensitivity and Specificity
  • Serologic Tests / methods*
  • Vaccination / statistics & numerical data
  • Zika Virus Infection / diagnosis

Substances

  • Antibodies, Viral
  • Dengue Vaccines
  • Immunoglobulin G
  • Immunoglobulin M