Development of a simple and rapid immunochromatographic strip test for detecting duck plague virus antibodies based on gI protein

J Virol Methods. 2020 Mar:277:113803. doi: 10.1016/j.jviromet.2019.113803. Epub 2019 Dec 19.

Abstract

A colloidal gold strip (CGS) for detecting antibodies to duck plague virus (DPV) was developed. Colloidal gold-labeled DPV gI protein and goat anti-rabbit IgG were dispensed on a conjugate pad as tracers. The recombinant DPV gI protein and rabbit IgG were used as capture reagents at the test line and control line, respectively. The detection limit of this assay was 1:256. Additionally, the CGS did not react with antisera from other common duck diseases, only reacting with anti-DPV serum and yielding a specific and strong red signal. 123 serum samples were tested by CGS and enzyme-linked immunosorbent assay (ELISA), and the results showed good agreement. The CGS test results can be observed in 15 min with the naked eye, should be suitable for clinical testing and large-scale detection.

Keywords: Colloidal gold strip (CGS); DPV gI protein; Duck plague virus.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Ducks / virology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Gold Colloid
  • Herpesviridae Infections / diagnosis
  • Herpesviridae Infections / immunology
  • Herpesviridae Infections / veterinary*
  • Immunoassay / instrumentation
  • Immunoassay / methods*
  • Mardivirus / immunology*
  • Neutralization Tests / methods
  • Reagent Strips
  • Recombinant Proteins
  • Sensitivity and Specificity
  • Viral Proteins / immunology*

Substances

  • Antibodies, Viral
  • Gold Colloid
  • Reagent Strips
  • Recombinant Proteins
  • Viral Proteins