A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7

Front Cell Infect Microbiol. 2019 Dec 4:9:417. doi: 10.3389/fcimb.2019.00417. eCollection 2019.

Abstract

B1-type human adenoviruses (HAdVs) HAdV-3, HAdV-7, and HAdV-55 have caused epidemics in North America, Asia, and Europe. However, to date, no adenovirus vaccines or antiviral drugs have been approved for general use. In the present work, a scFv-phage immune library was constructed and mouse monoclonal antibody (MMAb) 10G12 was obtained through selection. 10G12 is specific for HAdV-7 and binds the hexon loop1 and loop2 (LP12), resulting in strong neutralization activity against HAdV-7. Additionally, it is stable in serum and buffer at various pH values. The findings provide insight into adenovirus and antibody responses and may facilitate the design and development of adenovirus vaccines and antiviral drugs.

Keywords: antiviral drugs; human adenovirus type 7 (HAdV-7); immune library; mouse monoclonal antibody (MMAb); neutralizing antibody.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Infections, Human / immunology*
  • Adenovirus Infections, Human / virology
  • Adenoviruses, Human / immunology*
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Neutralizing / immunology*
  • Antibodies, Viral / immunology*
  • Cell Line, Tumor
  • Cell Surface Display Techniques
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Female
  • Host-Pathogen Interactions / immunology
  • Humans
  • Hydrogen-Ion Concentration
  • Mice
  • Neutralization Tests
  • Single-Chain Antibodies / immunology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Single-Chain Antibodies