Hsa-let-7b Suppresses Cell Proliferation by Targeting UHRF1 in Melanoma

Cancer Invest. 2020 Jan;38(1):52-60. doi: 10.1080/07357907.2019.1709482. Epub 2020 Jan 20.

Abstract

UHRF1 promotes melanoma progression by inducing cell proliferation, and is correlated with poor prognosis of melanoma patients. However, the regulation mechanism has not been fully elaborated. Here, we detected hsa-let-7b expression and its role in melanoma. Through Targetscan and miRanda predication, 30 overlapped miRNAs were found; further survival analysis revealed that hsa-let-7b was the only miRNA that affected the overall survival. Overexpressed hsa-let-7b could significantly inhibit the proliferation ability of A375 and A2058 cells, and this phenomenon was reversed after co-transfection with pLenti-UHRF1. In conclusion, hsa-let-7b regulates melanoma cells proliferation in vitro by targeting UHRF1.

Keywords: Hsa-let-7b; UHRF1; melanoma; prognosis; proliferation.

MeSH terms

  • CCAAT-Enhancer-Binding Proteins / genetics*
  • Cell Proliferation / genetics
  • Datasets as Topic
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • Melanoma / genetics*
  • Melanoma / mortality
  • Melanoma / pathology
  • MicroRNAs / metabolism*
  • Middle Aged
  • Skin / pathology
  • Skin Neoplasms / genetics*
  • Skin Neoplasms / mortality
  • Skin Neoplasms / pathology
  • Survival Analysis
  • Ubiquitin-Protein Ligases / genetics*

Substances

  • CCAAT-Enhancer-Binding Proteins
  • MicroRNAs
  • mirnlet7 microRNA, human
  • UHRF1 protein, human
  • Ubiquitin-Protein Ligases