Histology of ex vivo skin after treatment with fractionated picosecond Nd:YAG laser in high and low-energy settings

J Cosmet Laser Ther. 2020;22(1):43-47. doi: 10.1080/14764172.2019.1710536. Epub 2020 Jan 3.

Abstract

Background: The fractionated picosecond laser produces microscopic lesions in the epidermis and dermis, which are known as laser-induced optical breakdown (LIOB) and intra-dermal laser-induced cavitation (LIC). There have been multiple histological reports on these phenomena, although some have been challenged on the grounds of similarity to artifacts. Asian skins, with a higher melanin content, may react differently to this treatment, and present literature is also lacking in this area.Purpose: To observe and report the histological effect of different energy levels and parameters of the fractional 532 nm/1064 nm picosecond laser on Asian skin ex vivo.Methods: Six skin samples were taken from clinically normal-looking perilesional areas and treated with different energy levels and parameters of the fractional 532 nm/1064 nm picosecond laser. The specimens were then sent to the lab for H&E staining, and the slides were reviewed by a dermatopathologist.Results: Superficial, intra-epidermal LIOBs were seen in skin treating at higher laser energies; deep, intra-dermal LICs were seen in skin treated at lower energies. Lesion sizes and depths were consistent with previously reported values on Caucasian skins, and lesions were spaced in 600-μm intervals or its multiple.Conclusions: The histological findings are consistent with results from other ethnicities, and the spacing of lesions is a strong indication of their validity as LIOBs or LICs.

Keywords: Picosecond laser; fractional picosecond laser; intra-dermal LIC; intra-epidermal LIOB; laser induced cavitation; laser induced optical breakdown.

MeSH terms

  • Dermatologic Surgical Procedures / methods
  • Epidermis / pathology
  • Humans
  • Lasers, Solid-State / therapeutic use*
  • Low-Level Light Therapy / methods*
  • Models, Biological
  • Skin / pathology
  • Skin Aging / radiation effects*
  • Skin Physiological Phenomena / radiation effects*