Molecular characterization of carbapenem-resistant Klebsiella pneumoniae ST14 and ST512 causing bloodstream infections in ICU and surgery wards of a tertiary university hospital of Verona (northern Italy): co-production of KPC-3, OXA-48, and CTX-M-15 β-lactamases

Alessandra Piccirilli; Mariagrazia Perilli; Valentina Piccirilli; Bernardetta Segatore; Gianfranco Amicosante; Laura Maccacaro; Alda Bazaj; Laura Naso; Giuliana Lo Cascio; Giuseppe Cornaglia

doi:10.1016/j.diagmicrobio.2019.114968

Molecular characterization of carbapenem-resistant Klebsiella pneumoniae ST14 and ST512 causing bloodstream infections in ICU and surgery wards of a tertiary university hospital of Verona (northern Italy): co-production of KPC-3, OXA-48, and CTX-M-15 β-lactamases

Diagn Microbiol Infect Dis. 2020 Mar;96(3):114968. doi: 10.1016/j.diagmicrobio.2019.114968. Epub 2019 Dec 13.

Affiliations

¹ Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy.
² Dipartimento di Scienze Cliniche Applicate e Biotecnologiche, Università degli Studi dell'Aquila, L'Aquila, Italy. Electronic address: perilli@univaq.it.
³ U.O.C. di Microbiologia e Virologia, Dipartimento di Patologia e Diagnostica, Azienda Ospedaliera Universitaria Integrata di Verona, Verona, Italy.
⁴ Dipartimento di Diagnostica e Sanità Pubblica, Università di Verona, Verona, Italy.

PMID: 31924425
DOI: 10.1016/j.diagmicrobio.2019.114968

Abstract

Klebsiella pneumoniae strain is an important opportunistic pathogen that causes severe nosocomial infections. In the present study a molecular characterization of carbapenem resistant K. pneumoniae, isolated from blood samples of hospitalized patients of Verona University Hospital, was performed. The simultaneous presence of SHV-1/CTX-M-15/KPC-3 and SHV-1/CTX-M-15/OXA-48 serin-β-lactamases was ascertained in the 89% and 11% of K. pneumoniae ST512 and K. pneumoniae ST14, respectively. Molecular characterization of bla genes showed that bla_KPC-3 was found in Tn4401a transposon with the tnpR, tnpA, ISKpn6, and ISKpn7 mobile elements whereas bla_CTX-M-15 was detected downstream ISEcp1 genetic element. A class 1 integron with a gene cassette of 780 bp corresponding to aadA2 gene was identified in 33 K. pneumoniae ST512 isolates.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / biosynthesis*
Bacterial Proteins / genetics
Drug Resistance, Multiple, Bacterial
Genetic Variation
Hospitals, University / statistics & numerical data
Humans
Intensive Care Units / statistics & numerical data
Italy
Klebsiella Infections / blood*
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics*
Microbial Sensitivity Tests
Patients' Rooms / statistics & numerical data
Tertiary Care Centers / statistics & numerical data*
beta-Lactamases / biosynthesis*
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-lactamase CTX-M-15
beta-Lactamases
beta-lactamase KPC-3, Klebsiella pneumoniae
oxacillinase