Formation of bioactive peptides during simulated gastrointestinal digestion is affected by αs1-casein polymorphism in buffalo milk

Shanshan Li; Qi Hu; Chen Chen; Jianxin Liu; Guoqing He; Ling Li; Jianping Wu; Daxi Ren

doi:10.1016/j.foodchem.2020.126159

Formation of bioactive peptides during simulated gastrointestinal digestion is affected by α_s1-casein polymorphism in buffalo milk

Food Chem. 2020 May 30:313:126159. doi: 10.1016/j.foodchem.2020.126159. Epub 2020 Jan 7.

Authors

Shanshan Li¹, Qi Hu², Chen Chen³, Jianxin Liu³, Guoqing He², Ling Li⁴, Jianping Wu⁵, Daxi Ren⁶

Affiliations

¹ Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; ZJU-UA Joint Lab for Molecular Nutrition and Bioactive Peptides, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
² Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China; ZJU-UA Joint Lab for Molecular Nutrition and Bioactive Peptides, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
³ Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
⁴ Water Buffalo Institute, Chinese Academy of Agricultural Science, Nanning 530001, China.
⁵ ZJU-UA Joint Lab for Molecular Nutrition and Bioactive Peptides, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Department of Agricultural, Food and Nutritional Science, 4-10 Ag/For Building, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Electronic address: jwu3@ualberta.ca.
⁶ Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: dxren@zju.edu.cn.

PMID: 31931427
DOI: 10.1016/j.foodchem.2020.126159

Abstract

The polymorphism of buffalo α_s1-casein has been reported, but little is known about their effect on the biological properties. The objective of this study was to investigate the effect of α_s1-casein polymorphism on the digestive properties and bioactivities of buffalo milk protein in simulated gastrointestinal digestion. In this study, two variants of α_s1-casein, with one amino acid substitution of Leu193 (AA) → Ser193 (BB), were used. Under acidic gastric conditions, the particle size of α_s1-casein variant BB was smaller and showed higher digestibility compared to variant AA. A total of 154 and 149 peptides were identified, respectively, from simulated gastrointestinal digestion of variants AA and BB; of three peptides have been previously reported to exert ACE-inhibition, anticancer, antioxidant, and anxiolytic activities. Our study demonstrated that α_s1-casein polymorphism affects the digestive properties and the formation of bioactive peptides.

Keywords: Bioactive peptides; Buffalo milk; Gastrointestinal digestion; α(s1)-casein polymorphism.

MeSH terms

Amino Acid Substitution
Animals
Buffaloes*
Caseins / genetics*
Caseins / metabolism
Caseins / pharmacokinetics*
Digestion / drug effects
Humans
Milk / chemistry
Milk / physiology*
Milk Proteins / analysis
Particle Size
Peptides / metabolism*
Polymorphism, Genetic*

Substances

Caseins
Milk Proteins
Peptides