Generation of a hiPSC line ZZUNEUi007-A from a patient with hypertrophic cardiomyopathy caused by mutation in MYH7

Xiaowei Li; Yangyang Liu; Feifei Liu; Xiaojuan Wang; Mengduan Liu; Wenting Du; Jing Zhao; Mengyu Wang; Liang Hu; Chuchu Wang; Wanrong Fu; Jianzeng Dong; Xiaoyan Zhao

doi:10.1016/j.scr.2020.101699

Generation of a hiPSC line ZZUNEUi007-A from a patient with hypertrophic cardiomyopathy caused by mutation in MYH7

Stem Cell Res. 2020 Mar:43:101699. doi: 10.1016/j.scr.2020.101699. Epub 2020 Jan 7.

Authors

Xiaowei Li¹, Yangyang Liu¹, Feifei Liu², Xiaojuan Wang³, Mengduan Liu¹, Wenting Du¹, Jing Zhao¹, Mengyu Wang¹, Liang Hu¹, Chuchu Wang⁴, Wanrong Fu¹, Jianzeng Dong⁵, Xiaoyan Zhao⁶

Affiliations

¹ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China.
² Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
³ Department of Obstetrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
⁴ School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China.
⁵ Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, National Clinical Research Centre for Cardiovascular Diseases, No. 2 Beijing Anzhen Road, Chaoyang District, Beijing 100029, China; Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China; Department of Obstetrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: jz_dong@126.com.
⁶ Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China. Electronic address: fcczhaoxy8@zzu.edu.cn.

PMID: 31931472
DOI: 10.1016/j.scr.2020.101699

Abstract

Hypertrophic cardiomyopathy (HCM) is the most common monogenic cardiovascular disorder. In this study, we generated human induced pluripotent stem cells (iPSC) ZZUNEUi007-A from dermal fibroblasts of an HCM patient with the p. R663H (c. 1988 G > A) mutation in MYH7. The generated hiPSC line had normal karyotype, showed robust expression of pluripotency markers and could differentiate into all three germ layers in vivo.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cardiac Myosins / genetics*
Cardiomyopathy, Hypertrophic / genetics*
Female
Humans
Induced Pluripotent Stem Cells / metabolism*
Mutation
Myosin Heavy Chains / genetics*

Substances

MYH7 protein, human
Cardiac Myosins
Myosin Heavy Chains