The role and difference of TLR2 and TLR4 in rhabdomyolysis induced acute kidney injury in mice

Zongze He; Jijun Li; Yan Mei; Yang Lv; Fei Zhu; Ran Liu; Xu Wang; Fenghua Yao; Li Zhang; Xiangmei Chen

The role and difference of TLR2 and TLR4 in rhabdomyolysis induced acute kidney injury in mice

Int J Clin Exp Pathol. 2018 Feb 1;11(2):1054-1061. eCollection 2018.

Authors

Zongze He^{1

2}, Jijun Li², Yan Mei¹, Yang Lv¹, Fei Zhu¹, Ran Liu¹, Xu Wang¹, Fenghua Yao², Li Zhang¹, Xiangmei Chen¹

Affiliations

¹ Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases Beijing 100853, China.
² The First Hospital Affiliated to The Chinese PLA General Hospital Beijing, China.

PMID: 31938201
PMCID: PMC6958052

Abstract

To investigate the role of toll like receptors (TLRs) 2 and 4 in rhabdomyolysis (RM)-related acute kidney injury (AKI). Wild-type (WT) mice and TLR2 knockout (TLR2^-/-) or TLR4 knockout (TLR4^-/-) mice were injected with either saline (sham) or glycerin (to induce RM-related AKI). Samples were collected for detection of 0 h 24 h (Cr) creatinine, urea nitrogen (BUN), creatine kinase (CK), and PAS staining of renal tissues. Serum Cr and BUN level was significantly increased in TLR2^-/- and TLR4^-/-AKI groups more than those in the control group and the WT mice in AKI group. TLR4^-/-AKI group Cr, BUN level, and the pathological damage was lightest. The expression levels of signal transduction proteins in TLR2^-/- and TLR4^-/-AKI group were higher than in the control group, but was lower than that in the wild AKI group, with the TLR4^-/-AKI group having the lowest levels. The expression level of inflammatory factor mRNA in TLR2^-/- and TLR4^-/-AKI groups was higher than that in control group, but was lower than that in wild AKI group, with TLR4^-/-AKI group displaying lowest levels. Knockout of TLRs 2 and 4 decreased kidney inflammation and improved RM-related AKI.

Keywords: Toll like receptor 4; acute kidney injury; inflammation; rhabdomyolysis; toll like receptor 2.