Identification of Reversible Small Molecule Inhibitors of Endothelial Lipase (EL) That Demonstrate HDL-C Increase In Vivo

J Med Chem. 2020 Feb 27;63(4):1660-1670. doi: 10.1021/acs.jmedchem.9b01831. Epub 2020 Feb 11.

Abstract

Endothelial lipase (EL) hydrolyzes phospholipids in high-density lipoprotein (HDL) resulting in reduction in plasma HDL levels. Studies with murine transgenic, KO, or loss-of-function variants strongly suggest that inhibition of EL will lead to sustained plasma high-density lipoprotein cholesterol (HDL-C) increase and, potentially, a reduced cardiovascular disease (CVD) risk. Herein, we describe the discovery of a series of oxadiazole ketones, which upon optimization, led to the identification of compound 12. Compound 12 was evaluated in a mouse pharmacodynamics (PD) model and demonstrated a 56% increase in plasma HDL-C. In a mouse reverse cholesterol transport study, compound 12 stimulated cholesterol efflux by 53% demonstrating HDL-C functionality.

MeSH terms

  • Animals
  • Cholesterol, HDL / metabolism*
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / pharmacokinetics
  • Enzyme Inhibitors / pharmacology*
  • Ketones / chemical synthesis
  • Ketones / pharmacokinetics
  • Ketones / pharmacology*
  • Lipase / antagonists & inhibitors*
  • Male
  • Mice, Inbred C57BL
  • Molecular Structure
  • Oxadiazoles / chemical synthesis
  • Oxadiazoles / pharmacokinetics
  • Oxadiazoles / pharmacology*
  • Structure-Activity Relationship

Substances

  • Cholesterol, HDL
  • Enzyme Inhibitors
  • Ketones
  • Oxadiazoles
  • Lipase
  • Lipg protein, mouse