First Draft Genome of the Trypanosomatid Herpetomonas muscarum ingenoplastis through MinION Oxford Nanopore Technology and Illumina Sequencing

Claudia M d'Avila-Levy; Bertrand Bearzatto; Jérôme Ambroise; Raphaël Helaers; Anzhelika Butenko; Vyacheslav Yurchenko; Karina A Morelli; Helena L C Santos; Pascal Brouillard; Philippe Grellier; Jean-Luc Gala; Miikka Vikkula

doi:10.3390/tropicalmed5010025

First Draft Genome of the Trypanosomatid Herpetomonas muscarum ingenoplastis through MinION Oxford Nanopore Technology and Illumina Sequencing

Trop Med Infect Dis. 2020 Feb 13;5(1):25. doi: 10.3390/tropicalmed5010025.

Authors

Affiliations

¹ Coleção de Protozoários, Laboratório de Estudos Integrados em Protozoologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-360, Brazil.
² de Duve Institute, University of Louvain, B-1200 Brussels, Belgium.
³ Centre de Technologies Moléculaires Appliquées, Université Catholique de Louvain, B-1200 Brussels, Belgium.
⁴ Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 37005 České Budějovice (Budweis), Czech Republic.
⁵ Life Science Research Centre, Faculty of Science, University of Ostrava, 71000 Ostrava, Czech Republic.
⁶ Martsinovsky Institute of Medical Parasitology, Sechenov University, 119435 Moscow, Russia.
⁷ Instituto de Biologia Roberto Alcântara Gomes, Departamento de Ecologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-900, Brazil.
⁸ Unité Molécules de Communication et Adaptation des Microorganisme (UMR 7245 CNRS MCAM), Muséum National d'Histoire Naturelle, Sorbonne Universités, 75005 Paris, France.

Abstract

Here, we present first draft genome sequence of the trypanosomatid Herpetomonas muscarum ingenoplastis. This parasite was isolated repeatedly in the black blowfly, Phormia regina, and it forms a phylogenetically distinct clade in the Trypanosomatidae family.

Keywords: Trypanosomatidae; genome assembly; insect trypanosomatids; monoxenous trypanosomatids; whole genome.