Shotgun proteomic analysis of ESBL-producing and non-ESBL-producing Klebsiella Pneumoniae clinical isolates

Shymaa Enany; Samira Zakeer; Ahmed A Sayed; Sameh Magdeldin

doi:10.1016/j.micres.2020.126423

Shotgun proteomic analysis of ESBL-producing and non-ESBL-producing Klebsiella Pneumoniae clinical isolates

Microbiol Res. 2020 Jan 25:234:126423. doi: 10.1016/j.micres.2020.126423. Online ahead of print.

Authors

Shymaa Enany¹, Samira Zakeer², Ahmed A Sayed³, Sameh Magdeldin⁴

Affiliations

¹ Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Egypt. Electronic address: shymaa21@yahoo.com.
² Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Egypt.
³ Genomic Research Program, Basic Research Department, Children's Cancer Hospital Egypt 57357, 11441 Cairo, Egypt; Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
⁴ Proteomics and Metabolomics Unit, Department of Basic Research, Children's Cancer Hospital Egypt 57357, 11441 Cairo, Egypt; Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, 41522 Ismailia, Egypt.

PMID: 32078895
DOI: 10.1016/j.micres.2020.126423

Abstract

Klebsiella pneumoniae is a pathogenic bacterium that is responsible for a wide range of infections in humans. An increased rate of infections caused by multi-drug-resistant K. pneumoniae has been noted in the last two decades. The association between antimicrobial resistance and virulence is an important topic of study. Genomic tools have been used widely for the detection of virulence. In our study, we used proteomic analysis with mass spectrometry and bioinformatics tools to explore the virulence factors of both ESBL-producing and non-ESBL-producing K. pneumoniae and to determine the association between virulence and antimicrobial resistance in these clinical isolates. We have revealed different proteomic profiles and different pathways between the ESBL- and non-ESBL-producing groups. Many proteins involved in stress responses have been reported in the shared proteome between ESBL-and non-ESBL producers, such as ElaB protein, Lon protease, and universal stress proteins G and A. The virulence and pathogenicity of ESBL-producing bacteria were stronger than those of the non-ESBL-producing bacteria. Several unique virulence determinants were identified in ESBL-producing K. pneumoniae, such as proteins with lyase, catalase, isochorismatase, and oxidoreductase activity.

Keywords: ESBLs; Klebsiella pneumoniae; Proteomics.