Association between variants of the autophagy related gene ATG16L1 in inflammatory bowel diseases and clinical statues

Shaghayegh Baradaran Ghavami; Fateme Kabiri; Mahyar Nourian; Hedieh Balaii; Shabnam Shahrokh; Vahid Chaleshi; Ghazal Sherkat; Farzaneh Shalileh; Hamid Asadzadeh Aghdaei

Association between variants of the autophagy related gene ATG16L1 in inflammatory bowel diseases and clinical statues

Gastroenterol Hepatol Bed Bench. 2019;12(Suppl1):S94-S100.

Authors

Shaghayegh Baradaran Ghavami¹, Fateme Kabiri¹, Mahyar Nourian², Hedieh Balaii², Shabnam Shahrokh², Vahid Chaleshi², Ghazal Sherkat³, Farzaneh Shalileh², Hamid Asadzadeh Aghdaei²

Affiliations

¹ Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
³ Student Research Committee, Islamic Azad University, Mashhad Branch, Mashhad, Iran.

PMID: 32099608
PMCID: PMC7011055

Abstract

Aim: In the present study, two main variants of ATG16L1 gene, rs2241880 T300A and rs2241879 C/T, were evaluated in IBD patients as well as in remission and flareup phase across an Iranian population for the first time.

Background: Inflammatory bowel disease (IBD) has found increasing global incidence and prevalence in recent years especially among pediatrics. ATG16L1 is the major gene that regulates autophagy pathway. The autophagy pathway also affects dysbiosis.

Methods: Genomic DNA was isolated from peripheral blood samples following salting out extraction method. The genotypes of ATG16L1 polymorphisms rs2241880 T300A and rs2241879 C/T were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

Results: In this case control study, a total of 101 IBD patients (75 ulcerative colitis (UC) and 26 Crohn's disease (CD)) and 99 healthy controls were evaluated. In the present study, a significant association was found between rs2241879 single nucleotide polymorphism on ATG16L1 gene and increased risk of IBD among an Iranian population (P=0.01). There was no statistically significant relationship between rs2241880 and IBD risk (P= 0.42). The effect on these two variants was investigated in relapse and flareup phase which was not significant either, but in CD, rs2241879 and rs2241880 were difference in the relapse phase.

Conclusion: The results showed that ATG16L1 gene rs2241879 has a significant relationship with increased risk of IBD among an Iranian population. Individuals with C allele showed a significant relationship with 1.68-fold increased risk of IBD (P=0.01; adjusted OR=1.68; 95% CI=1.13-2.50).

Keywords: ATG16L1; Autophagy; Diseases status; Inflammatory bowel disease.