Rapid mesoscale volumetric imaging of neural activity with synaptic resolution

Rongwen Lu; Yajie Liang; Guanghan Meng; Pengcheng Zhou; Karel Svoboda; Liam Paninski; Na Ji

doi:10.1038/s41592-020-0760-9

Rapid mesoscale volumetric imaging of neural activity with synaptic resolution

Nat Methods. 2020 Mar;17(3):291-294. doi: 10.1038/s41592-020-0760-9. Epub 2020 Mar 2.

Authors

Rongwen Lu^#^{1

2}, Yajie Liang^#¹, Guanghan Meng^#³, Pengcheng Zhou^{4

5

6}, Karel Svoboda¹, Liam Paninski^{4

5

6}, Na Ji^{7

8

9

10

11}

Affiliations

¹ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
² National Eye Institute, National Institutes of Health, Bethesda, MD, USA.
³ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
⁴ Department of Statistics, Columbia University, New York, NY, USA.
⁵ Department of Neuroscience, Columbia University, New York, NY, USA.
⁶ Zuckerman Institute, Columbia University, New York, NY, USA.
⁷ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA. jina@berkeley.edu.
⁸ Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. jina@berkeley.edu.
⁹ Department of Physics, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹⁰ Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA. jina@berkeley.edu.
¹¹ Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. jina@berkeley.edu.

^# Contributed equally.

Abstract

Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over the mesoscale with synaptic resolution. We applied the technology to calcium imaging of entire dendritic spans of neurons as well as neural ensembles within multiple cortical regions over two hemispheres of the awake mouse brain.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Algorithms
Animals
Brain / physiology*
Calcium / chemistry
Dendrites / physiology*
Female
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence, Multiphoton / methods*
Models, Neurological
Neurons / physiology*
Radiosurgery
Synapses / physiology*
gamma-Aminobutyric Acid

Substances

gamma-Aminobutyric Acid
Calcium

Abstract

Publication types

MeSH terms

Substances

Grants and funding