Vascular progenitors generated from tankyrase inhibitor-regulated naïve diabetic human iPSC potentiate efficient revascularization of ischemic retina

Nat Commun. 2020 Mar 5;11(1):1195. doi: 10.1038/s41467-020-14764-5.

Abstract

Here, we report that the functionality of vascular progenitors (VP) generated from normal and disease-primed conventional human induced pluripotent stem cells (hiPSC) can be significantly improved by reversion to a tankyrase inhibitor-regulated human naïve epiblast-like pluripotent state. Naïve diabetic vascular progenitors (N-DVP) differentiated from patient-specific naïve diabetic hiPSC (N-DhiPSC) possessed higher vascular functionality, maintained greater genomic stability, harbored decreased lineage-primed gene expression, and were more efficient in migrating to and re-vascularizing the deep neural layers of the ischemic retina than isogenic diabetic vascular progenitors (DVP). These findings suggest that reprogramming to a stable naïve human pluripotent stem cell state may effectively erase dysfunctional epigenetic donor cell memory or disease-associated aberrations in patient-specific hiPSC. More broadly, tankyrase inhibitor-regulated naïve hiPSC (N-hiPSC) represent a class of human stem cells with high epigenetic plasticity, improved multi-lineage functionality, and potentially high impact for regenerative medicine.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adult
  • Animals
  • Blood Vessels / pathology*
  • Cell Differentiation / drug effects
  • Cell Line
  • Cell Lineage / drug effects
  • Cell Movement / drug effects
  • Cellular Senescence / drug effects
  • DNA Damage
  • Diabetes Mellitus / pathology*
  • Enzyme Inhibitors / pharmacology
  • Epigenesis, Genetic / drug effects
  • Fibroblasts / drug effects
  • Fibroblasts / pathology
  • Histone Code
  • Humans
  • Induced Pluripotent Stem Cells / drug effects
  • Induced Pluripotent Stem Cells / pathology*
  • Ischemia / pathology
  • Ischemia / therapy*
  • Mice
  • Organoids / drug effects
  • Organoids / pathology
  • Poly(ADP-ribose) Polymerase Inhibitors / pharmacology
  • Promoter Regions, Genetic / genetics
  • Retina / pathology*
  • Stem Cells / drug effects
  • Stem Cells / pathology*
  • Stem Cells / ultrastructure
  • Tankyrases / antagonists & inhibitors*
  • Tankyrases / metabolism
  • Teratoma / pathology
  • Transcription, Genetic / drug effects

Substances

  • Enzyme Inhibitors
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Tankyrases