RYBP/YAF2-PRC1 complexes and histone H1-dependent chromatin compaction mediate propagation of H2AK119ub1 during cell division

Nat Cell Biol. 2020 Apr;22(4):439-452. doi: 10.1038/s41556-020-0484-1. Epub 2020 Mar 23.

Abstract

Stable propagation of epigenetic information is important for maintaining cell identity in multicellular organisms. However, it remains largely unknown how mono-ubiquitinated histone H2A on lysine 119 (H2AK119ub1) is established and stably propagated during cell division. In this study, we found that the proteins RYBP and YAF2 each specifically bind H2AK119ub1 to recruit the RYBP-PRC1 or YAF2-PRC1 complex to catalyse the ubiquitination of H2A on neighbouring nucleosomes through a positive-feedback model. Additionally, we demonstrated that histone H1-compacted chromatin enhances the distal propagation of H2AK119ub1, thereby reinforcing the inheritance of H2AK119ub1 during cell division. Moreover, we showed that either disruption of RYBP/YAF2-PRC1 activity or impairment of histone H1-dependent chromatin compaction resulted in a significant defect of the maintenance of H2AK119ub1. Therefore, our results suggest that histone H1-dependent chromatin compaction plays a critical role in the stable propagation of H2AK119ub1 by RYBP/YAF2-PRC1 during cell division.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Cell Division
  • Epigenesis, Genetic
  • Feedback, Physiological
  • Gene Deletion
  • Gene Editing
  • HEK293 Cells
  • Histones / genetics*
  • Histones / metabolism
  • Humans
  • Mice
  • Mouse Embryonic Stem Cells / cytology
  • Mouse Embryonic Stem Cells / metabolism*
  • Muscle Proteins / genetics*
  • Muscle Proteins / metabolism
  • Nucleosomes / chemistry
  • Nucleosomes / metabolism
  • Polycomb Repressive Complex 1 / genetics*
  • Polycomb Repressive Complex 1 / metabolism
  • Protein Binding
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Protein Processing, Post-Translational*
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Ubiquitination

Substances

  • Cell Cycle Proteins
  • Histones
  • Muscle Proteins
  • Nucleosomes
  • Protein Isoforms
  • Repressor Proteins
  • Rybp protein, mouse
  • Yaf2 protein, mouse
  • Polycomb Repressive Complex 1