DNA-binding of zinc(II) and nickel(II) salphen-like complexes extrapolated at 1 M salt concentration: Removing the ionic strength bias in physiological conditions

J Inorg Biochem. 2020 Jun:207:111064. doi: 10.1016/j.jinorgbio.2020.111064. Epub 2020 Mar 17.

Abstract

The DNA-binding of two salphen-like metal complexes of nickel(II) (1) and zinc(II) (2) was investigated in different ionic strength solutions by absorption spectroscopy. The data analysis allowed us to obtain the values of their extrapolated DNA-binding constant in physiological conditions, with DNA-binding strength in the order Ni > Zn, and to give relative weight to the electrostatic and non-electrostatic contributions to their DNA-interaction.

Keywords: DNA; Ionic strength; Nickel; Salphen; UV–visible absorption; Zinc.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coordination Complexes / chemistry*
  • DNA / chemistry*
  • Nickel / chemistry*
  • Osmolar Concentration
  • Phenylenediamines / chemistry*
  • Sodium Chloride / chemistry
  • Spectrum Analysis
  • Static Electricity
  • Zinc / chemistry*

Substances

  • Coordination Complexes
  • Phenylenediamines
  • salphen
  • Sodium Chloride
  • Nickel
  • DNA
  • Zinc