Identification of porcine reproductive and respiratory syndrome virus inhibitors through an oriented screening on natural products

Wen-Gui Li; Fei-Yan Dai; Yong-Xian Cheng; Ge-Fen Yin; Jun-Long Bi; Dian-Peng Li

doi:10.1007/s40242-013-2300-y

Identification of porcine reproductive and respiratory syndrome virus inhibitors through an oriented screening on natural products

Chem Res Chin Univ. 2013;29(2):290-293. doi: 10.1007/s40242-013-2300-y. Epub 2013 Mar 10.

Authors

Wen-Gui Li¹, Fei-Yan Dai¹, Yong-Xian Cheng², Ge-Fen Yin¹, Jun-Long Bi¹, Dian-Peng Li³

Affiliations

¹ 12300College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201 P. R. China.
² 22300State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204 P. R. China.
³ 32300Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin, 541006 P. R. China.

Abstract

Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide, causing big economic losses. Vaccines are major weapons against PRRSV, however, current available vaccines have several limitations. Developing chemical drugs as alternatives is required. On the basis of traditional medical knowledge, we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects. Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay. Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC₅₀) values of 7.9 and 39.4 μmol/L, respectively. Meanwhile, compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner. Study results suggest that compounds ethoxysanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization.

Keywords: Antiviral activity; Cytopathic effect; Natural product; Porcine reproductive and respiratory syndrome virus(PRRSV).