Thermodynamic Cost, Speed, Fluctuations, and Error Reduction of Biological Copy Machines

J Phys Chem Lett. 2020 Apr 16;11(8):3136-3143. doi: 10.1021/acs.jpclett.0c00545. Epub 2020 Apr 7.

Abstract

Due to large fluctuations in cellular environments, transfer of information in biological processes without regulation is error-prone. The mechanistic details of error-reducing mechanisms in biological copying processes have been a subject of active research; however, how error reduction of a process is balanced with its thermodynamic cost and dynamical properties remain largely unexplored. Here, we study the error reducing strategies in light of the recently discovered thermodynamic uncertainty relation (TUR) that sets a physical bound to the cost-precision trade-off for dissipative processes. We found that the two representative copying processes, DNA replication by the exonuclease-deficient T7 DNA polymerase and mRNA translation by the E. coli ribosome, reduce the error rates to biologically acceptable levels while also optimizing the processes close to the physical limit dictated by TUR.

MeSH terms

  • DNA Replication / physiology*
  • Models, Biological*
  • Protein Biosynthesis / physiology*
  • Thermodynamics*