Blood microRNA 202-3p associates with the risk of essential hypertension by targeting soluble ST2

Biosci Rep. 2020 May 29;40(5):BSR20200378. doi: 10.1042/BSR20200378.

Abstract

MicroRNA (miR)-202-3p has attracted a great deal of attention in the fields of oncology, gynecology, and metabolic disorders. However, its role in cardiovascular diseases remains to be clarified. We previously found that disruption of miR-202-3p mediated regulation of expression of soluble (s)ST2, a decoy receptor for interleukin (IL)-33, promotes essential hypertension (EH). In the present study, we first measured miR-202-3p expression levels in the blood of 182 EH cases and 159 healthy controls using TaqMan assays. miR-202-3p levels were shown to be significantly higher in EH cases than controls (fold change = 3.58, P<0.001). Logistic regression analysis revealed that higher miR-202-3p expression was associated with an increased occurrence of EH (adjusted odds ratio (OR): 1.57; 95% confidence interval (CI), 1.36-1.82; P<0.001). Addition of miR-202-3p to traditional risk factors showed an additive prediction value for EH. Further functional experiments indicated that miR-202-3p could be induced by angiotensin II (Ang II) and inhibited by Ang II-triggered soluble ST2 (sST2) expression in a negative feedback manner. Moreover, blood miR-202-3p levels were negatively correlated with sST2 expression in vivo. Our study shows that blood miR-202-3p levels were significantly associated with the occurrence of EH. These findings indicate that miR-202-3p exerts a protective role against EH by antagonizing the induction of sST2 by Ang II.

Keywords: essential hypertension; miR-202-3p; sST2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Angiotensin II / pharmacology
  • Biomarkers / blood
  • Blood Pressure*
  • Case-Control Studies
  • Circulating MicroRNA / blood*
  • Circulating MicroRNA / genetics
  • Essential Hypertension / blood*
  • Essential Hypertension / diagnosis
  • Essential Hypertension / genetics
  • Essential Hypertension / physiopathology
  • Feedback, Physiological
  • Female
  • Gene Expression Regulation
  • Humans
  • Interleukin-1 Receptor-Like 1 Protein / blood*
  • Interleukin-1 Receptor-Like 1 Protein / genetics
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / metabolism
  • Male
  • MicroRNAs / blood*
  • MicroRNAs / genetics
  • Middle Aged
  • THP-1 Cells

Substances

  • Biomarkers
  • Circulating MicroRNA
  • IL1RL1 protein, human
  • Interleukin-1 Receptor-Like 1 Protein
  • MIRN203 microRNA, human
  • MicroRNAs
  • Angiotensin II