Investigation of the human nasal microbiome in persons with long- and short-term exposure to methicillin-resistant Staphylococcus aureus and other bacteria from the pig farm environment

PLoS One. 2020 Apr 30;15(4):e0232456. doi: 10.1371/journal.pone.0232456. eCollection 2020.

Abstract

Since its emergence in the early 2000s, livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398) has led to an increasing number of human infections in Denmark and other European countries with industrial pig production. LA-MRSA CC398 is primarily associated with skin infections among pig farm workers but is also increasingly recognized as a cause of life-threatening disease among elderly and immunocompromised people. Pig farm workers may serve as vehicles for the spread of LA-MRSA CC398 and other farm-origin bacteria between farms and into the general population. Yet, little is known about the bacterial community dynamics in pig farm workers and other persons with long- and short-term exposure to the pig farm environment. To gain insight into this, we investigated the nasal microbiomes in pig farm workers during a workweek on four LA-MRSA CC398-positive pig farms, as well as in short-term visitors two hours before, immediately after, and 48 hours after a 1-hour visit to another LA-MRSA CC398-positive pig farm. S. aureus and LA-MRSA CC398 carriage was quantified by means of culture, and the composition of the bacterial communities was investigated through sequencing of the 16S rRNA gene. Pig farm workers often carried LA-MRSA CC398 and other bacteria from the pig farm environment, both at work and at home, although at lower levels at home. In contrast, short-term visitors were subject to a less dramatic and rapidly reversible change in the nasal bacterial community composition. These results suggest that pig farm workers may be an important source of LA-MRSA CC398 and perhaps other pathogens of human and veterinary relevance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Carrier State / epidemiology*
  • Carrier State / microbiology
  • Carrier State / transmission
  • DNA, Bacterial / isolation & purification
  • Farms / statistics & numerical data
  • Female
  • Humans
  • Male
  • Methicillin-Resistant Staphylococcus aureus / genetics
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification*
  • Microbiota / genetics
  • Middle Aged
  • Nose / microbiology
  • Occupational Exposure / adverse effects*
  • RNA, Ribosomal, 16S / genetics
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / transmission
  • Swine / microbiology*
  • Time Factors
  • Young Adult

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S

Grants and funding

This study was supported by a grant from the Ministry of Environment and Food of Denmark through The Danish Agrifish Agency (J. no. 33010-NIFA-14-612 to ARL and JL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.