Mitochondrial genome sequence of Phytophthora sansomeana and comparative analysis of Phytophthora mitochondrial genomes

PLoS One. 2020 May 14;15(5):e0231296. doi: 10.1371/journal.pone.0231296. eCollection 2020.

Abstract

Phytophthora sansomeana infects soybean and causes root rot. It was recently separated from the species complex P. megasperma sensu lato. In this study, we sequenced and annotated its complete mitochondrial genome and compared it to that of nine other Phytophthora species. The genome was assembled into a circular molecule of 39,618 bp with a 22.03% G+C content. Forty-two protein coding genes, 25 tRNA genes and two rRNA genes were annotated in this genome. The protein coding genes include 14 genes in the respiratory complexes, four ATP synthase genes, 16 ribosomal proteins genes, a tatC translocase gene, six conserved ORFs and a unique orf402. The tRNA genes encode tRNAs for 19 amino acids. Comparison among mitochondrial genomes of 10 Phytophthora species revealed three inversions, each covering multiple genes. These genomes were conserved in gene content with few exceptions. A 3' truncated atp9 gene was found in P. nicotianae. All 10 Phytophthora species, as well as other oomycetes and stramenopiles, lacked tRNA genes for threonine in their mitochondria. Phylogenomic analysis using the mitochondrial genomes supported or enhanced previous findings of the phylogeny of Phytophthora spp.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • DNA, Mitochondrial / genetics*
  • Evolution, Molecular*
  • Genome, Mitochondrial*
  • Mitochondria / genetics*
  • Mitochondrial Proteins / genetics*
  • Phylogeny
  • Phytophthora / classification*
  • Phytophthora / genetics*
  • Sequence Homology
  • Species Specificity

Substances

  • DNA, Mitochondrial
  • Mitochondrial Proteins

Grants and funding

This study was supported by the United States Department of Agriculture. The authors, as USDA employees, performed study design, data collection and analysis, and manuscript preparation.