Minicircle DNA: The Future for DNA-Based Vectors?

Ana Margarida Almeida; João António Queiroz; Fani Sousa; Ângela Sousa

doi:10.1016/j.tibtech.2020.04.008

Minicircle DNA: The Future for DNA-Based Vectors?

Trends Biotechnol. 2020 Oct;38(10):1047-1051. doi: 10.1016/j.tibtech.2020.04.008. Epub 2020 May 11.

Authors

Ana Margarida Almeida¹, João António Queiroz¹, Fani Sousa¹, Ângela Sousa²

Affiliations

¹ CICS-UBI - Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.
² CICS-UBI - Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal. Electronic address: angela@fcsaude.ubi.pt.

PMID: 32409109
DOI: 10.1016/j.tibtech.2020.04.008

Abstract

Minicircle DNA (mcDNA) is a smaller and safer version of non-viral DNA vectors that results from a cutting-edge in vivo recombination process to excise prokaryotic sequences from plasmid DNA (pDNA). Considering the molecule's potential and increasing interest as a non-viral DNA-based therapeutic, biomanufacturing methodologies need to be improved, especially in downstream processing.

Keywords: downstream processing; gene delivery; minicircle DNA biomanufacturing; plasmid DNA; upstream processing.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biotechnology / methods*
DNA*
Genetic Vectors*
Plasmids*

Substances

DNA