Procedures for Culturing and Genetically Manipulating Murine Hippocampal Postnatal Neurons

Enora Moutin; Anne-Laure Hemonnot; Vincent Seube; Nathalie Linck; François Rassendren; Julie Perroy; Vincent Compan

doi:10.3389/fnsyn.2020.00019

Procedures for Culturing and Genetically Manipulating Murine Hippocampal Postnatal Neurons

Front Synaptic Neurosci. 2020 Apr 30:12:19. doi: 10.3389/fnsyn.2020.00019. eCollection 2020.

Authors

Enora Moutin¹, Anne-Laure Hemonnot^{1

2}, Vincent Seube^{1

2}, Nathalie Linck^{1

2}, François Rassendren^{1

2}, Julie Perroy¹, Vincent Compan^{1

2}

Affiliations

¹ Institut de Génomique Fonctionnelle (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France.
² Laboratoire d'Excellence Canaux Ioniques d'Intérêt Thérapeutique (LabEx ICST), Montpellier, France.

Abstract

Neuronal hippocampal cultures are simple and valuable models for studying neuronal function. While embryonic cultures are widely used for different applications, mouse postnatal cultures are still challenging, lack reproducibility and/or exhibit inappropriate neuronal activity. Yet, postnatal cultures have major advantages such as allowing genotyping of pups before culture and reducing the number of experimental animals. Herein we describe a simple and fast protocol for culturing and genetically manipulating hippocampal neurons from P0 to P3 mice. This protocol provides reproducible cultures exhibiting a consistent neuronal development, normal excitatory over inhibitory neuronal ratio and a physiological neuronal activity. We also describe simple and efficient procedures for genetic manipulation of neurons using transfection reagent or lentiviral particles. Overall, this method provides a detailed and validated protocol allowing to explore cellular mechanisms and neuronal activity in postnatal hippocampal neurons in culture.

Keywords: culture; hippocampus; lentivirus; neurons; postnatal; transduction; transfection.