The Apolipoprotein A-I Mimetic L-4F Attenuates Monocyte Activation and Adverse Cardiac Remodeling after Myocardial Infarction

Int J Mol Sci. 2020 May 15;21(10):3519. doi: 10.3390/ijms21103519.

Abstract

Excessive inflammation after myocardial infarction (MI) can promote infarct expansion and adverse left ventricular (LV) remodeling. L-4F, a mimetic peptide of apolipoprotein A-I (apoA-I), exhibits anti-inflammatory and anti-atherogenic properties; however, whether L-4F imparts beneficial effects after myocardial infarction (MI) is unknown. Here we demonstrate that L-4F suppresses the expansion of blood, splenic, and myocardial pro-inflammatory monocytes and macrophages in a mouse model of reperfused MI. Changes in immune cell profiles were accompanied by alleviation of post-MI LV remodeling and dysfunction. In vitro, L-4F also inhibited pro-inflammatory and glycolytic gene expression in macrophages. In summary, L-4F treatment prevents prolonged and excessive inflammation after MI, in part through modulation of pro-inflammatory monocytes and macrophages, and improves post-MI LV remodeling. These data suggest that L-4F could be a used as a therapeutic adjunct in humans with MI to limit inflammation and alleviate the progression to heart failure.

Keywords: cardiac remodeling; heart failure; inflammation; macrophage polarity.

MeSH terms

  • Animals
  • Apolipoprotein A-I / metabolism*
  • Cell Plasticity / drug effects
  • Inflammation / pathology
  • Macrophage Activation / drug effects
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Macrophages / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Monocytes / drug effects
  • Monocytes / pathology*
  • Myocardial Infarction / physiopathology*
  • Peptides / pharmacology*
  • RAW 264.7 Cells
  • Systole / drug effects
  • Ventricular Dysfunction, Left / physiopathology
  • Ventricular Remodeling / drug effects*

Substances

  • Apolipoprotein A-I
  • L-4F peptide
  • Peptides