Determination of carnitine ester profile in the children with type 1 diabetes: a valuable step towards a better management

Arch Physiol Biochem. 2022 Oct;128(5):1209-1214. doi: 10.1080/13813455.2020.1762662. Epub 2020 May 24.

Abstract

Objective: This study is designed to investigate the levels of carnitine and acylcarnitines (ACs) in the children with diabetes type 1 compared to the healthy subjects.Methods: Forty-two type 1 diabetic children and healthy subjects were recruited in the study, respectively. In addition to FBS and Hb A1C, free carnitine and ACs in butyl-ester form in the fasting blood samples were assessed by isotope dilution mass spectrometry for all diabetics and controls using the tandem mass spectrometry system.Results: Diabetic patients had a higher level of C, C4, C6, C14, C18:2, and C18:2OH. Females had elevated C14:2 compared to the males. The C18:2 and C18:2OH levels were elevated as the Hb A1C level increased. The C18:2, C14OH were mostly increased in the prediabetic and diabetic patients, respectively.Conclusion: Increased ACs level indicates the increased acyl-CoA intermediates for the fatty acids and amino acids oxidation.

Keywords: Acyl carnitine; children; free carnitine; metabolism; type 1 diabetes.

MeSH terms

  • Amino Acids
  • Carnitine / analogs & derivatives
  • Carnitine / metabolism
  • Child
  • Coenzyme A
  • Diabetes Mellitus, Type 1*
  • Esters
  • Fatty Acids / metabolism
  • Female
  • Glycated Hemoglobin
  • Humans
  • Isotopes
  • Male

Substances

  • Amino Acids
  • Esters
  • Fatty Acids
  • Glycated Hemoglobin A
  • Isotopes
  • acylcarnitine
  • Carnitine
  • Coenzyme A