Pathogenic variants in EP300 and ANKRD11 in patients with phenotypes overlapping Cornelia de Lange syndrome

Am J Med Genet A. 2020 Jul;182(7):1690-1696. doi: 10.1002/ajmg.a.61611. Epub 2020 May 31.

Abstract

Cornelia de Lange syndrome (CdLS), Rubinstein-Taybi syndrome (RSTS), and KBG syndrome are three distinct developmental human disorders. Variants in seven genes belonging to the cohesin pathway, NIPBL, SMC1A, SMC3, HDAC8, RAD21, ANKRD11, and BRD4, were identified in about 80% of patients with CdLS, suggesting that additional causative genes remain to be discovered. Two genes, CREBBP and EP300, have been associated with RSTS, whereas KBG results from variants in ANKRD11. By exome sequencing, a genetic cause was elucidated in two patients with clinical diagnosis of CdLS but without variants in known CdLS genes. In particular, genetic variants in EP300 and ANKRD11 were identified in the two patients with CdLS. EP300 and ANKRD11 pathogenic variants caused the reduction of the respective proteins suggesting that their low levels contribute to CdLS-like phenotype. These findings highlight the clinical overlap between CdLS, RSTS, and KBG and support the notion that these rare disorders are linked to abnormal chromatin remodeling, which in turn affects the transcriptional machinery.

Keywords: Cornelia de Lange syndrome; KBG syndrome; Rubinstein-Taybi syndrome; chromatin remodeling; transcription.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abnormalities, Multiple / etiology
  • Bone Diseases, Developmental / etiology
  • Child
  • Child, Preschool
  • De Lange Syndrome / etiology*
  • De Lange Syndrome / genetics
  • E1A-Associated p300 Protein / genetics*
  • Exome Sequencing
  • Facies
  • Female
  • Genetic Variation
  • Humans
  • Infant
  • Intellectual Disability / etiology
  • Male
  • Repressor Proteins / genetics*
  • Rubinstein-Taybi Syndrome / etiology
  • Tooth Abnormalities / etiology

Substances

  • ANKRD11 protein, human
  • Repressor Proteins
  • E1A-Associated p300 Protein
  • EP300 protein, human

Supplementary concepts

  • KBG syndrome