Elevated temperature increases the accumulation of microbial necromass nitrogen in soil via increasing microbial turnover

Abstract

Microbial-derived nitrogen (N) is now recognized as an important source of soil organic N. However, the mechanisms that govern the production of microbial necromass N, its turnover, and stabilization in soil remain poorly understood. To assess the effects of elevated temperature on bacterial and fungal necromass N production, turnover, and stabilization, we incubated ¹⁵ N-labeled bacterial and fungal necromass under optimum moisture conditions at 10°C, 15°C, and 25°C. We developed a new ¹⁵ N tracing model to calculate the production and mineralization rates of necromass N. Our results showed that bacterial and fungal necromass N had similar mineralization rates, despite their contrasting chemistry. Most bacterial and fungal necromass ¹⁵ N was recovered in the mineral-associated organic matter fraction through microbial anabolism, suggesting that mineral association plays an important role in stabilizing necromass N in soil, independently of necromass chemistry. Elevated temperature significantly increased the accumulation of necromass N in soil, due to the relatively higher microbial turnover and production of necromass N with increasing temperature than the increases in microbial necromass N mineralization. In conclusion, we found elevated temperature may increase the contribution of microbial necromass N to mineral-stabilized soil organic N.

Keywords: elevated temperature; global climate changes; microbial necromass; nitrogen stable isotopes; soil modeling; soil nitrogen; soil organic matter.