A novel mitochondria-targeted ratiometric fluorescent probe for endogenous sulfur dioxide derivatives as a cancer-detecting tool

J Mater Chem B. 2020 Jul 14;8(26):5722-5728. doi: 10.1039/d0tb00149j. Epub 2020 Jun 9.

Abstract

A new mitochondria-targeted fluorescent probe RBC, constructed using a coumarin moiety which was selected as the donor and a benzothiazole derivative as the acceptor, for SO2 derivatives (HSO3-/SO32-) was presented. The probe designed on a new FRET platform showed high selectivity and a low detection limit. Importantly, the probe could respond to HSO3-/SO32- within 35 s. Furthermore, the probe could target mitochondria and was successfully used for fluorescence imaging of endogenous bisulfite in HepG2 with low cytotoxicity, which significantly assisted in cancer diagnosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles / chemistry
  • Benzothiazoles / pharmacology*
  • Cells, Cultured
  • Coumarins / chemical synthesis
  • Coumarins / chemistry
  • Coumarins / pharmacology*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / pharmacology*
  • Humans
  • Hydrogen-Ion Concentration
  • Mitochondria / drug effects*
  • Molecular Structure
  • Optical Imaging
  • Particle Size
  • Sulfur Dioxide / analysis*
  • Surface Properties

Substances

  • Benzothiazoles
  • Coumarins
  • Fluorescent Dyes
  • Sulfur Dioxide
  • coumarin
  • benzothiazole