Abstract
We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.
MeSH terms
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Enzyme Assays*
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / pharmacology
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Hydrolysis
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular*
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Nucleotides / chemistry
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Nucleotides / metabolism
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Phosphoric Diester Hydrolases / analysis*
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Phosphoric Diester Hydrolases / metabolism
Substances
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Enzyme Inhibitors
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Nucleotides
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Phosphoric Diester Hydrolases